Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line1 Scope This document describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264. 7 macrophage cells exposed to nano objects, nanoparticles, their aggregates and agglomerates using the CM H2DCFDA assay. The protocol in this document is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

c or 1 500 V d

IEC 60947-4-1:2000

and oil exploration and production units

BS EN 14908-3 establishes a minimal set of rules for compliance

so that selection may be made to produce an overall design

BS EN 2743 provides you with the standard procedures for conditioning prior to testing unaged fibre reinforced plastic materials including reinforcing fibres and cured resin systems for aerospace applications

1 The main applications of the lessons learned

Although the focus of this document is on providing information on updates

complying with ISO 9801

Pulse calibration

are not covered by this European Standard

Metal engineers