Animal feeding stuffs: Methods of sampling and analysis. Determination of ergot alkaloids and tropane alkaloids in feed materials and compound feeds by LC-MS/MS1 Scope This document describes a method for the determination of individual ergot alkaloids and tropane alkaloids in unprocessed cereals and cereal based compound feeds by high performance liquid chromatography with tandem mass spectrometry (LC MS MS). This document has been successfully validated by collaborative trial in the following matrices: rye, barley, wheat, complete feed for bovine, porcine and poultry. Validation in buckwheat produced

Size designation and dimensions

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The reference to safety requirements according to EN 60065 has been deleted

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